Effects of macrophage activity on the antibody-dependent cytotoxicity against Trichinella spiralis newborn larvae: an in vitro cytotoxicity and ultrastructural study.

The effect of the activity of macrophages on the antibody-dependent cytotoxicity against Trichinella spiralis newborn larvae was studied in vitro. Macrophages present in peritoneal exudates from mice genetically selected for high and low antibody production (HL and LL, respectively) showed an inverse cytotoxic effect. Cells from HL mice were ineffective, whereas cells from LL mice had a very high killing capacity.

[Filaria medinensis: differential serodiagnosis using ELISA].

An immunoenzymatic technic ELISA has been used to permit the diagnosis of Dracunculus medinensis . In effect an immunological tool to differentiate helminthiasis, particularly filariosis does not exist yet. For this purpose we have begun to prepare an homologous antigen of a filariosis species ( Dracunculus medinensis ).

Trichinella spiralis infection in animals genetically selected for high and low antibody production. Studies on intestinal pathology.

Specific antibody response and histopathological changes in the small intestine were studied in mice genetically selected for high and low antibody production (HL and LL, respectively) after oral infection with 50 muscle larvae of Trichinella spiralis. Specific antibody response, measured during the first 20 days after infection in the passive cutaneous anaphylaxis assay and the immunofluorescence test was high in HL and low in LL mice. The response of macrophages, intestinal mast cells, globule leucocytes and eosinophils was examined in the jejunum.