Functional surfaces for exosomes capturing and exosomal microRNAs analysis.

Exosomes are small extracellular vesicles well-studied both as cell signaling elements and as source of highly informative biomarkers, in particular microRNAs. Standard techniques for exosome isolation are in general scarcely efficient and give low purity vesicles. New techniques combining microfluidics with suitable functionalized surfaces could overcome these disadvantages.

Mother-to-Infant Microbial Transmission from Different Body Sites Shapes the Developing Infant Gut Microbiome.

The acquisition and development of the infant microbiome are key to establishing a healthy host-microbiome symbiosis. The maternal microbial reservoir is thought to play a crucial role in this process. However, the source and transmission routes of the infant pioneering microbes are poorly understood.

The Iceman's lithic toolkit: Raw material, technology, typology and use.

The Tyrolean Iceman, a 5,300-year-old glacier mummy recovered at the Tisenjoch (South Tyrol, Italy) together with his clothes and personal equipment, represents a unique opportunity for prehistoric research. The present work examines the Iceman's tools which are made from chert or are related to chert working - dagger, two arrowheads, endscraper, borer, small flake and antler retoucher - and considers also the arrowhead still embedded in the shoulder of the mummy. The interdisciplinary results achieved by study of the lithic raw material, technology, use-wear analysis, CT analysis and typology all add new information to Ötzi's individual history and his last days, and allow insights into the way of life of Alpine Copper Age communities.

Maternal inheritance of bifidobacterial communities and bifidophages in infants through vertical transmission.

Background: The correct establishment of the human gut microbiota represents a crucial development that commences at birth. Different hypotheses propose that the infant gut microbiota is derived from, among other sources, the mother's fecal/vaginal microbiota and human milk.

Results: The composition of bifidobacterial communities of 25 mother-infant pairs was investigated based on an internal transcribed spacer (ITS) approach, combined with cultivation-mediated and genomic analyses.