[The use of lateral central electroanalgesia in patients with borderline mental disturbances].

Results are reported on the use of lateral central electroanalgesia (current frequency: 1500-2000 Hz) in 59 patients with borderline mental disorders characterized by frequent protracted attacks. It was found that maximum reduction of psychopathological symptoms was observed in patients with somatic and senestopathic hypochondriac subdepressions, hypoparanoid structures of hypervaluable level in the limits of small progredient schizophrenia and atypical cyclotomy.

Anionic subsites of the acetylcholinesterase from Torpedo californica: affinity labelling with the cationic reagent N,N-dimethyl-2-phenyl-aziridinium.

Several peptides of acetylcholinesterase of Torpedo californica labelled with the alkylating reagent [3H]N,N-dimethyl-2-phenyl-aziridinium (DPA) were localized within the primary structure. One peptide had the sequence KPQELIDVE (positions 270-278); the incorporation of DPA into this peptide could be specifically suppressed by propidium, which suggests that it is part of the peripheral anionic site. The incorporation of DPA into two other peptides was insensitive to propidium but could be prevented by edrophonium; the sequence of one of the peptides assumed to be part of the anionic site in the catalytic centre was found to be DLFR (positions 217-220).

Acetylcholinesterase as polyelectrolyte in reaction with cationic substrates.

It is shown that the salt effect in acetylcholinesterase-catalyzed hydrolysis of 2-(N-methylmorpholinium)-ethylacetate can be quantitatively described by the equation log(k2/KS) = log(k2/KS) degrees--psi log[M+Z] following from Manning's polyelectrolyte theory; the psi values for salts with univalent and bivalent cations at different pH values of the reaction medium were in accordance with the conclusions of the theory. Manning's polyelectrolyte theory seems to be a useful framework for studying salt effects in the reactions of charged substrates with enzymes as globular polyions.

The muscarinic receptor-mediated action of acetylcholine in the gastrulating chick embryo.

Some of the muscarinic receptor-mediated effects of acetylcholine in early chick embryo cells at stages three-four by Hamburger and Hamilton were studied. Acetylcholine increased the intracellular level of cGMP about two-fold. Acetylcholine raised the intracellular level of free calcium from the basal level of 120 nM to 140 nM.