Publications by authors named "A Pardo-Vargas"
Article Synopsis
- Microbial glycan degradation plays a crucial role in global carbon cycling, with the marine bacterium Salegentibacter sp. Hel_I_6 showing potential in breaking down α-mannan, a component sourced from fungi.
- The bacterium's gene cluster includes an endo-α-1,6-mannanase enzyme (ShGH76) that functions similarly to enzymes found in human gut bacteria, indicating a shared ability to digest fungal material.
- Research findings show that ShGH76 has unique structural characteristics and demonstrates strong activity on α-mannan substrates, hinting at the presence of previously unidentified fungal α-1,6-mannans in marine ecosystems, particularly during periods of microalgae blooms.
Article Synopsis
- Carbohydrates like oligosaccharides and polysaccharides are important biopolymers involved in various biological processes and often studied after being isolated from natural sources.
- Pure compounds with well-defined structures, obtained via chemical or enzymatic synthesis, are necessary for accurate analysis, and new synthetic techniques have made it easier to access larger polysaccharides.
- The study uses advanced MALDI FT-ICR mass spectrometry to analyze synthetic carbohydrates of different sizes, revealing details about their monosaccharide content and linkage types through the identification of fragment ions, enhancing our understanding of their composition and structures.
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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- * The study uses cryogenic vibrational spectroscopy and ion mobility-mass spectrometry to analyze B-type fragments from protected galactosides made from different glycosyl donors.
- * Results show that the infrared signatures of these fragments indicate they have the same structure, implying that fully protected monosaccharides do not retain anomeric memory in B-type ions.
Imaging of biomolecules guides our understanding of their diverse structures and functions. Real-space imaging at sub-nanometre resolution using cryo-electron microscopy has provided key insights into proteins and their assemblies. Direct molecular imaging of glycans-the predominant biopolymers on Earth, with a plethora of structural and biological functions-has not been possible so far.
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