Photonic platform coupled with machine learning algorithms to detect pyrolysis products of crack cocaine in saliva: A proof-of-concept animal study.

The non-invasive detection of crack/cocaine and other bioactive compounds from its pyrolysis in saliva can provide an alternative for drug analysis in forensic toxicology. Therefore, a highly sensitive, fast, reagent-free, and sustainable approach with a non-invasive specimen is relevant in public health. In this animal model study, we evaluated the effects of exposure to smoke crack cocaine on salivary flow, salivary gland weight, and salivary composition using Attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy.

Ancient genomes reveal a deep history of treponemal disease in the Americas.

Human treponemal infections are caused by a family of closely related Treponema pallidum that give rise to the diseases yaws, bejel, pinta and, most famously, syphilis. Debates on both a common origin for these pathogens and the history of syphilis itself has weighed evidence for the "Columbian hypothesis", which argues for an American origin, against that for the "pre-Columbian hypothesis", which argues for presence of the disease in Eurasia in the Medieval period and possibly earlier. While molecular data has provided a genetic basis for distinction of the typed subspecies, deep evolution of the complex has remained unresolved due to limitations in the conclusions that can be drawn from the sparse paleogenomic data currently available.

Intensifying intermittent aeration for optimizing nutrient and hormone removal in vertical-flow constructed wetlands filled with aerated concrete.

Operational strategies have been applied in constructed wetlands to optimize the removal of nutrients and hormones that are still a concern in wastewater treatment. The strategy of intensifying intermittent aeration was investigated in two microcosm-scale vertical-flow constructed wetlands (VFCWs) planted with Eichhornia crassipes onto autoclaved aerated concrete (AC) in the removal of nutrients, estrone (E1), 17β-estradiol (E2) and 17α-ethinylestradiol (EE2). CW-1 (2.

Pumpkin seed protein as a carrier for Astaxanthin: Molecular characterization of interactions and implications for stability.

This study investigated pumpkin seed protein (PSP) as a carrier for astaxanthin (AST). Interaction mechanisms revealed through fluorescence spectroscopy and molecular docking, showed that hydrogen bonds and Van der Waals forces form the PSP-AST complex. AST binding altered PSP's secondary structure, increasing α-helix (7.

Occurrence of Leishmania spp. DNA and specific antibodies in dogs from Acre State, Rio Branco, Brazil.

Canine leishmaniasis is a parasitic disease whose agents are transmitted through the bites of infected phlebotomine sand flies. This disease is endemic in tropical and subtropical regions, including Brazil. However, information on its prevalence in dogs in some Brazilian states remains limited.