An immunological approach to prion diseases.

Ovine scrapie and bovine spongiform encephalopathy are genetic diseases, presenting probably autoimmunity transmissible by the oral route. The absence of immune response in prion diseases indicates a tolerant state for PrP(C) and PrP(SC). The tolerant state against these diseases should be overcome before immunizing animals.

Mice ascites as a source of polyclonal and monoclonal antibodies.

A large quantity of polyclonal anti-ovalbumin antibodies was obtained from mice by a simple modification of the method described by Kurpisz et al. (1988). In addition, the cells from ascitic fluid were used to produce monoclonal antibodies.

Common antigenic properties of a g-type (goose) and a c-type (duck) egg white lysozyme: antibody responses in rabbits and mice.

Embden goose (GEWL) and Barbary duck (DEWL) egg white lysozymes possess different amino acid sequences corresponding to the g-type and c-type, respectively. GEWL was shown to be a better immunogen than DEWL in both rabbits and mice. The antigenicity of the two lysozymes was tested using different techniques (i.