Publications by authors named "A P van Leeuwen"
The study of somatic mutations in single cells provides insights into aging and carcinogenesis, which is complicated by the dependency on whole-genome amplification (WGA). Here, we describe a detailed workflow starting from single-cell isolation to WGA by primary template-directed amplification (PTA), sequencing, quality control, and downstream analyses. A machine learning approach, the PTA Analysis Toolkit (PTATO), is used to filter the hundreds to thousands of artificial variants induced by WGA from true mutations at high sensitivity and accuracy.
View Article and Find Full Text PDFChildhood is an obvious period for motor learning, since children's musculoskeletal and nervous systems are still in development. Adults adapt movements based on reward feedback about success and failure, but it is less established whether school-age children also exhibit such reward-based motor learning. We designed a new 'circle-drawing' task suitable for assessing reward-based motor learning in both children (7-17 years old) and adults (18-65 years old).
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- Endothelial disorders leading to edema and poor tissue oxygenation are common in cardiac surgery with CPB, prompting researchers to investigate the protective effects of albumin as a component in the CPB circuit.
- In a study with male rats, CPB primed with LR/albumin/mannitol resulted in lower pulmonary edema compared to 6% hydroxyethyl starch (HES) despite similar renal edema levels, indicating potential benefits of using albumin in surgical settings.
- Although microcirculatory perfusion was impaired during CPB for both groups and did not improve post-CPB, the LR/albumin/mannitol group needed significantly less fluid and medication to maintain adequate blood flow, alongside showing reduced inflammatory markers
Background: Focal laser ablation (FLA) serves as a targeted therapy for prostate cancer (PCa). Clinical studies have demonstrated significant variations in ablation volumes with consistent fiber configurations. Consequently, a prediction model is needed for the safe application of FLA in treating PCa.
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- Shallow coastal and estuarine habitats are crucial for fish species, providing areas for spawning, nursery, and feeding, but these habitats are threatened by human activities that degrade their quality and availability.
- Different fish species utilize these habitats at various life stages, leading to their classification into functional guilds, which experience the impacts of habitat changes differently.
- Using size-structured population models, the study examines how reduced food productivity in estuaries affects two guilds: estuarine residents and marine estuarine-dependent species, finding that while overall fish biomass declines, juvenile populations of the latter can increase under certain conditions due to shifts in resource competition and availability.