The present article deals with the technology of obtaining decellularized cell-free collagen-based scaffolds from arterial vessels and surgical assessment of the possibility of experimentally implanting them into the blood system of laboratory animals for experimental purposes. The study was performed on arterial vessels (n=60) and fragments of the human internal thoracic artery (n=20). Described herein is a method of obtaining a connective-tissue matrix of a blood vessel by means of vessel's perfusion for 2-3 hours with detergent solutions.
View Article and Find Full Text PDFDuring the elaboration of an instrumental reflex, it is not obligatory to use a conditioned stimulus, which signals the necessity to generate an instrumental reaction in order to receive reinforcement. However, the presence of a conditioned stimulus simplifies analysis of instrumental reaction, which in this case is the response to the conditioned stimulus. On the other hand, it is necessary to distinguish between instrumental and classical conditioning, since in both cases the response to a conditioned stimulus increases.
View Article and Find Full Text PDFAt present it is not clear how instrumental actions arise. In our experiments the mollusk Helix received an aversive stimulus when one of its neurons did not generate an action potential in response to a conditioned stimulus. The appearance of an aversive stimulus did not depend on the generation or failure of a spike in the control neuron.
View Article and Find Full Text PDFGaining insight into the mechanism of generation of goal-directed actions is understanding neural function. In this study we examined the role of the action potential (AP) in a single molluscan neuron (responsible for a defensive response) in an instrumental behavior. The intracellular electrical activity of two neurons was recorded simultaneously.
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