Genetic and therapeutic landscapes in cohort of pancreatic adenocarcinomas: next-generation sequencing and machine learning for full tumor exome analysis.

About 7% of all cancer deaths are caused by pancreatic cancer (PCa). PCa is known for its lowest survival rates among all oncological diseases and heterogenic molecular profile. Enormous amount of genetic changes, including somatic mutations, exceeds the limits of routine clinical genetic laboratory tests and further stagnates the development of personalized treatments.

[Clinical, morphological, and prognostic features of papillary thyroid carcinoma with different BRAF mutational status assessed by immunohistochemistry].

Unlabelled: The somatic mutation in BRAF (BRAF), the data on the prognostic role of which are contradictory, is one of the most common molecular genetic abnormalities in the cells of papillary thyroid carcinoma (PTC).

Objective: To investigate the association of the immunoexpression of mutant BRAF in PTC with different morphological characteristics and long-term treatment results in patients.

Material And Methods: Information on inpatients with PTC was obtained from the database of the Pathology Department, A.

The effect of long-term -80°C storage of thyroid biospecimens on RNA quality and ensuring fitness for purpose.

Aims: To establish whether RNA degrades in long-term storage at -80°C and whether RNA integrity numbers (RINs) determine 'fitness for purpose' in severely degraded RNA.

Methods: RNA was extracted from 549 thyroid biospecimens stored at -80°C for 0.1-10.

[High-grade thyroid cancer].

The paper analyzes the data available in the literature on the morphological and prognostic features of relatively rare high-grade thyroid cancer. It shows the difficulties and unsolved problems of morphological diagnosis and the possibilities of current methods for immunohistochemical and molecular genetic studies to determine the malignant potential of the tumor and prognosis.

[A rare case of concurrent follicular and columnar cell variants of papillary thyroid carcinoma].

The paper describes a rare case of concurrent two different histological (follicular and columnar cell) variants of papillary carcinoma in one thyroid with columnar cell metastases to the lymph nodes and femoral bone. There are morphological features of and differences in BRAF status in the cells of two variants of papillary thyroid carcinoma.