Effect of Selected Organic Solvents on Hydroxyl Radical-Dependent Light Emission in the Fe-EGTA-HO System.

Numerous compounds that are scavengers of hydroxyl radicals (•OH) in Fenton systems have low solubility in water. Therefore, they are dissolved in organic solvents to reach suitable concentrations in the reaction milieu of the Fenton system. However, these solvents may react with •OH and iron, leading to significant errors in the results.

Light Emission from Fe-EGTA-HO System Depends on the pH of the Reaction Milieu within the Range That May Occur in Cells of the Human Body.

A Fe-EGTA(ethylene glycol-bis (β-aminoethyl ether)-,,','-tetraacetic acid)-HO system emits photons, and quenching this chemiluminescence can be used for determination of anti-hydroxyl radical (•OH) activity of various compounds. The generation of •OH and light emission due to oxidative damage to EGTA may depend on the buffer and pH of the reaction milieu. In this study, we evaluated the effect of pH from 6.

Kidney Transplant Recipients Show Limited Lung Diffusion Capacity but Similar Hydrogen Peroxide Exhalation as Healthy Matched Volunteers: A Pilot Study.

Patients with end-stage chronic kidney disease show higher systemic oxidative stress and exhale more hydrogen peroxide (HO) than healthy controls. Kidney transplantation reduces oxidative stress and HO production by blood polymorphonuclear leukocytes (PMNs). Kidney transplant recipients (KTRs) may be predisposed to an impairment of lung diffusing capacity due to chronic inflammation.

Concentration Dependence of Anti- and Pro-Oxidant Activity of Polyphenols as Evaluated with a Light-Emitting Fe-Egta-HO System.

Hydroxyl radical (OH) scavenging and the regeneration of Fe may inhibit or enhance peroxidative damage induced by a Fenton system, respectively. Plant polyphenols reveal the afore-mentioned activities, and their cumulative net effect may determine anti- or pro-oxidant actions. We investigated the influence of 17 phenolics on ultra-weak photon emission (UPE) from a modified Fenton system (92.

Effect of Physiological Concentrations of Vitamin C on the Inhibitation of Hydroxyl Radical Induced Light Emission from Fe-EGTA-HO and Fe-EGTA-HO Systems In Vitro.

Ascorbic acid (AA) has antioxidant properties. However, in the presence of Fe/Fe ions and HO, it may behave as a pro-oxidant by accelerating and enhancing the formation of hydroxyl radicals (OH). Therefore, in this study we evaluated the effect of AA at concentrations of 1 to 200 µmol/L on OH-induced light emission (at a pH of 7.