Publications by authors named "A P Sane"
Single-strand breaks (SSBs) are one of the most commonly occurring endogenous lesions with the potential to give rise to cytotoxic double-strand breaks (DSBs) during DNA replication. To investigate how replication-dependent DSBs are repaired, we employed Cas9 nickase (nCas9) to generate site- and strand-specific nicks in the budding yeast genome. We found that nCas9-induced nicks are converted to mostly double-ended DSBs during S phase.
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- Control over flowering time is crucial for plant survival, and the TFL1/CEN/BFT genes play a significant role in delaying flowering to promote continued vegetative growth.
- Researchers identified seven banana genes (MCN1-7) in the TFL1/CEN/BFT family, which mostly show expression in specific plant growth phases and decrease right before flowering initiation in different varieties.
- The functional similarity of three of these genes (MCNs1-3) to the TFL1 gene in Arabidopsis suggests they help regulate vegetative growth and prevent premature flowering in bananas.
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- The study focused on the prevalence of septic shock among critically ill COVID-19 patients in Addis Ababa, finding that 39.3% experienced this condition, which significantly worsens healthcare outcomes.
- Key risk factors for septic shock included advanced age (≥60), long ICU stays (over 7 days), the need for invasive ventilation, and having chronic obstructive pulmonary disease (COPD). In contrast, diabetes and cardiovascular diseases were linked to a lower risk of septic shock.
- The findings highlight the urgent need for healthcare professionals to closely monitor these high-risk patients, as septic shock poses a serious concern for those severely affected by COVID-19.
Article Synopsis
- * The study specifically highlights chylomicron retention disease (CRD) and uses advanced models to explore gene defects, dysfunctional cellular processes, and the significance of specific proteins in lipid transport and cellular stress dynamics.
- * Additionally, genetically modified animal models have been important for investigating various factors affecting CRD, such as environmental influences, gender differences in disease progression, and developmental issues.
Single-strand breaks (SSBs) are one of the most common endogenous lesions and have the potential to give rise to cytotoxic double-strand breaks (DSBs) during DNA replication. To investigate the mechanism of replication fork collapse at SSBs and subsequent repair, we employed Cas9 nickase (nCas9) to generate site and strand-specific nicks in the budding yeast genome. We show that nCas9-induced nicks are converted to mostly double-ended DSBs during S-phase.
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