Viability and oxidative stress of dental pulp cells after indirect application of chemomechanical agents: An in vitro study.

Aim: This study evaluated the transdentinal cytotoxic effects of enzymatic agents (EA) for chemomechanical carious tissue removal on human dental pulp cells.

Methodology: The groups were based on the performed dentine treatments (n = 8): G1: Positive Control (PC - no treatment); G2: Negative Control (NC - 35% H O for 2 min); G3: Brix 3000™ (BX) for 30 s; G4: BX for 2 min; G5: Papacarie Duo™ (PD) for 30 s; G6: PD for 2 min. The cells were evaluated for viability (VB; MTT assay) and production of reactive oxygen species (ROS; DCFH-DA assay) and nitric oxide (NO; Griess reagent).

Roughness assessment of colored compomers: Results after an erosive-abrasive in vitro cycling test.

Background: The present study aimed to evaluate and compare the surface roughness of a colored compomer and a composite resin, after 15 days of erosive-abrasive cycling.

Material And Methods: The sample included ninety circular specimens, randomly divided (n = 10): G1 Berry, G2 Gold, G3 Pink, G4 Lemon, G5 Blue, G6 Silver, G7 Orange and G8 Green, referring to the different colors of compomer (Twinky Star®, VOCO, Germany) and G9 for composite resin (Z250®, 3M ESPE). The specimens were submerged in artificial saliva and stored at 37°C for 24 hours.

Antibacterial activity of Brazilian red propolis and in vitro evaluation of free radical production.

Objective: This study aimed to evaluate the antibacterial activity of crude Brazilian red propolis (BRP) extract against anaerobic bacteria involved in primary endodontic infection. Additionally, we evaluate the cell viability and free radical production of human dental pulp fibroblasts (HDPF) in direct contact with mineral trioxide aggregate (MTA) and BRP.

Design: The Minimum Inhibitory Concentration, Minimum Bactericidal Concentration (MIC, MBC) and Minimum Inhibitory Concentration of Biofilm (MICB) of BRP against anaerobic endodontic pathogens were determined.

Salivary and Dental Plaque Composition in Disabled Children Who Require Home Care: A Cross-sectional Investigation.

Aims: The purpose of this study was to compare salivary and dental plaque (DP) composition between disabled children who require home care (DCHC) and a control group (CG) and to correlate it with oral and systemic health factors.

Materials And Methods: This cross-sectional study included 15 DCHC and 15 healthy children (aged between 4 and 10 years). The caregivers answered a questionnaire on disease diagnosis, medical history, dental history, and oral hygiene routine.

Proliferation rate and expression of stem cells markers during expansion in primary culture of pulp cells.

The aim of the present study was to evaluate the proliferation rate and the expression of stem cells markers during expansion in primary culture of dental pulp stem cells (DPSCs), comparing different techniques (explant and enzymatic digestion), subject ages (up to 40 and over 40) and cell passages (#2, #5 and #8). DPSCs were isolated using either the enzymatic digestion (ED) or explant (EX) technique. The number of days needed for the cells to reach confluence was determined.