Transferrin Receptor (TfR)-mediated transcytosis across the blood-brain barrier (BBB) enables the uptake of bispecific therapeutic antibodies into the brain. At therapeutically relevant concentrations, bivalent binding to TfR appears to reduce the transcytosis efficiency by receptor crosslinking. In this study, we aimed to improve BBB transcytosis of symmetric antibodies through minimizing their ability to cause TfR crosslinking.
View Article and Find Full Text PDFCurr Opin Neurobiol
December 2024
Cryo-electron tomography (cryo-ET) visualizes natively preserved cellular ultrastructure at molecular resolution. Recent developments in sample preparation workflows and image processing tools enable growing applications of cryo-ET in cellular neurobiology. As such, cryo-ET is beginning to unravel the in situ macromolecular organization of neurons using samples of increasing complexity.
View Article and Find Full Text PDFUnlabelled: In recent years, nuts have gained importance because of their nutritional benefits in promoting health and preventing chronic diseases. Despite their recognized role as part of healthy dietary patterns, global consumption is below recommended levels, necessitating an investigation of factors influencing consumption and the impact of nutrition education.
Objective: To investigate the attitudes, knowledge and consumption habits of students in Serbia regarding the consumption of nuts and to examine the role and importance of food- or nutrition-related topics in academic curricula in promoting positive attitudes and habits regarding the consumption of nuts among pharmacy students.
Background/aims: This study examined the effects of a specially designed energy-restricted diet with alternate carbohydrate intake on body composition and cardiometabolic risk factors in overweight and obese adults. The aim was to assess whether the intervention could lead to significant weight loss, improve body composition, and reduce cardiometabolic risks.
Methods: Sixty-five participants (34 women, 31 men) with an average BMI of 31.