[The DS-DIF-KORINE kit to identify microorganisms corynebacterium, including C. diphtheriae].

The DS-DIF-KORINE kit make it possible to identify 20 types of corynebacteriae entered the Bergi identification guide (1997) including biovars gravis and mitis and belfanti version of diphtheria agent. The possibility to determine its toxigenic characteristics is also provided The test system is ready to be applied since it has all needed components to implement bacteriologic analysis in search of diphtheroids and diagnostics of diphtheria. The presented data demonstrate high diagnostic effectiveness of the new DS-DIF-KORINE kit which has the state registration in Roszdravnadzor and is adapted for spectrophotometer automatic pickup and included into Microbe-Automate and Microbe-2 programs.

[The actual issues of comparing the results of quantitative immune-enzyme tests].

The analysis was applied to the situation on national market of immune-enzyme diagnosticums with further quantitative reporting of results. The issues of coincidence and interpretation of results and causes of discrepancy are considered The notions of biologic ambiguity and metrological traceability are explained.

[Mapping of diagnostically important antigenic regions of major outer membrane protein (MOMP) from Chlamydia trachomatis using recombinant proteins with overlapping structures].

Amino acid sequence of the Chlamydia trachomatis major outer membrane protein (MOMP) was modeled using a series of the recombinant proteins containing six 100 aa fragments of MOMP overlapped in 30 aa. Testing of recombinant antigens in EIA showed that proteins containing MOMP fragments comprising 191-286 and 191-354 aa regions had the greatest activity in the reaction with anti- C. trachomatis positive serum samples.

[Antigenic activity of human herpes virus type 8 proteins coding orfK8.1 and orf65].

A peptide scanning of the sequences of human herpes virus type 8 (HHV-8) proteins coding orfK8.1 and orf65 was made. The antigenic activity of peptides was evaluated by ELISA.

[Diagnostic advantages of the test system "DS-EIA-HBsAg-0.01" for detection of HBV surface antigen].

The new highly sensitive test system "DS-EIA-HBsAg-0.01" (Priority Certificate No. 2006129019 of August 10, 2006) in detecting hepatitis B surface antigen (HBsAg) was assessed.