Enhancing Kidney Quality Assessment: Power Doppler During Normothermic Machine Perfusion.

Objectives: Marginal donor kidneys are increasingly used for transplantation to overcome organ shortage. This study aims to investigate the additional value of Power Doppler (PD) imaging in kidney quality assessment during normothermic machine perfusion (NMP).

Methods: Porcine kidneys (n = 22) retrieved from a local slaughterhouse underwent 2 h of NMP.

Unraveling the role of E. coli and calf intestinal alkaline phosphatase in calcium phosphate synthesis.

The enzyme-catalyzed synthesis of calcium phosphate is a promising method for producing calcium-based nanomaterials for biomedical applications. The purpose of this work was to determine the type of phosphate that forms when alkaline phosphatase catalyzes the reaction, and to identify the role of natural biopolymers in calcium phosphate formation. In this research, we analyzed calcium phosphates that were synthesized in the presence of alkaline phosphatase from either E.

Experimental Evaluation of the Effect of Degradation on the Mechanical Behavior and Morphometric Characteristics of Functionally Graded Polymer Scaffolds.

Bone transplantation ranks second worldwide among tissue prosthesis surgeries. Currently, one of the most promising approaches is regenerative medicine, which involves tissue engineering based on polymer scaffolds with biodegradable properties. Once implanted, scaffolds interact directly with the surrounding tissues and in a fairly aggressive environment, which causes biodegradation of the scaffold material.

Re-engineering of a carotenoid-binding protein based on NMR structure.

Recently, a number of message passing neural network (MPNN)-based methods have been introduced that, based on backbone atom coordinates, efficiently recover native amino acid sequences of proteins and predict modifications that result in better expressing, more soluble, and stable variants. However, usually, X-ray structures, or artificial structures generated by algorithms trained on X-ray structures, were employed to define target backbone conformations. Here, we show that commonly used algorithms ProteinMPNN and SolubleMPNN display low sequence recovery on structures determined using NMR.

Perspectives of Decision Support System TeleRehab in the Management of Post-Stroke Telerehabilitation.

Stroke is the main cause of disability among adults. Decision-making in stroke rehabilitation is increasingly complex; therefore, the use of decision support systems by healthcare providers is becoming a necessity. However, there is a significant lack of software for the management of post-stroke telerehabilitation (TR).