Publications by authors named "A P G Alcantara"
Supports coated with amino-hexyl and amino octyl have been prepared from glyoxyl agarose beads and compared in their performance with octyl-agarose to immobilize lipases A and B from Candida antarctica (CALA and CALB). Immobilization courses were similar using all supports, but enzyme release was more difficult using the amino-alkyl supports suggesting a mixed interfacial activation/ionic exchange immobilization. The enzyme activity and specificity (using p-nitrophenyl propionate, triacetin and both isomers of methyl mandelate) greatly depended on the support.
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- - The study focuses on isolating and characterizing oviduct fluid extracellular vesicles (coEV) in goats, which are important for events around conception, as they have been previously identified in other mammals but not in caprine species.
- - Two experiments were conducted: one to isolate coEV from individual goats or a pool of three, and the other to analyze coEV during different phases of the estrous cycle (follicular vs. luteal).
- - Findings showed that while the size distribution of coEV was similar regardless of isolation method, those collected during the luteal phase were larger, and the presence of specific proteins indicated they were exosomes with distinct characteristics.
The focus of the present work is to find the optimal conditions for the activation of agarose beads with divinyl sulfone (DVS). The reactivity of the vinyl sulfone groups in the support was checked by the support capacity to react with ethylamine; via elemental analysis. In addition, trypsin was used as a model enzyme to test the immobilization and stabilization capabilities of the different supports.
View Article and Find Full Text PDFA Unique Presentation of Bacterial Group G Streptococcus Myopericarditis.
J Investig Med High Impact Case Rep
October 2024
Perimyocarditis and myopericarditis are inflammatory conditions of the pericardium and myocardium, often of idiopathic or infectious etiology, with viral infections being the most common. Nonrheumatic streptococcal myopericarditis (NSM) is a rare condition that can mimic acute myocardial infarction. This case report presents a 22-year-old male with no prior medical history who developed NSM following a streptococcal pharyngitis infection.
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