Publications by authors named "A P D Carvalho-Assef"
Objectives: Infections due to carbapenemase-producing Enterobacterales harboring more than one carbapenemase-encoding gene spreads mainly by plasmid and transposon mobilization.
Objectives: Analyze the mobile genetic elements carrying bla and bla of K. pneumoniae carbapenemase co-producers (KpKN).
Klebsiella spp. is an opportunistic pathogen which poses a significant threat to public health, especially due to antimicrobial resistance and biofilm formation. This study aimed to determine the antibiotic resistance profile, biofilm formation and β-lactamases production in Klebsiella spp.
View Article and Find Full Text PDFGenomic study of strains co-harboring and reveals a large multidrug-resistant plasmid encoding these carbapenemases in Brazil.
Front Microbiol
July 2024
Introduction: contributes significantly to the global issue of multidrug-resistant (MDR) nosocomial infections. Often, these strains demonstrate resistance to carbapenems (MDR-CRAB), the first-line treatment for infections instigated by MDR . Our study focused on the antimicrobial susceptibility and genomic sequences related to plasmids from 12 clinical isolates of that carry both the blaOXA-58 and carbapenemase genes.
View Article and Find Full Text PDFA 7-Year Brazilian National Perspective on Plasmid-Mediated Carbapenem Resistance in Enterobacterales, Pseudomonas aeruginosa, and Acinetobacter baumannii Complex and the Impact of the Coronavirus Disease 2019 Pandemic on Their Occurrence.
Clin Infect Dis
July 2023
Article Synopsis
- Carbapenemase production is a significant global health concern, and analyzing antimicrobial resistance (AMR) data is essential for effective public health policies, as showcased in this study focused on Brazil's AMR Surveillance Network.
- The study evaluated carbapenemase detection rates in Brazilian hospitals from 2015 to 2022, finding trends such as a notable increase in blaNDM detection and a decrease in blaKPC for Enterobacterales, particularly after the onset of the COVID-19 pandemic.
- Overall, the findings highlight the resilience of the AMR Surveillance Network and indicate a concerning shift in carbapenemase profiles, with blaNDM becoming increasingly prevalent.
Evaluation of phenotypic detection of carbapenemase-producing Pseudomonas spp. from clinical isolates.
Braz J Microbiol
March 2023
Article Synopsis
- Carbapenems are crucial last-resort antibiotics for treating infections from multidrug-resistant Gram-negative bacteria, but resistance through mechanisms like OprD loss and carbapenemases is a growing global concern.
- A study in Brazil tested the effectiveness of three phenotypic tests—Carba NP, Blue Carba, and mCIM/eCIM—to detect carbapenemase-producing Pseudomonas species, using 127 clinical isolates.
- Results showed that Carba NP had the highest sensitivity and successfully differentiated between carbapenemases types, while Blue Carba struggled with class B detection, highlighting the need for improved and more reliable resistance detection methods.