[Fluorescent probes for examination of spermatozoa in cryoprotective mediums].

By fluorescent spectroscopy and microscopy methods the possibility of fluorescent probes DSM, E-176, 3-DAB and FME application for study of cryoprotective agents' influence on the dog spermatozoa are investigated. It is established that FME and 3-DAD dyes are suitable for the posed problem solving, and the DSM and E-176 probes have restrictions owing to enough strong fluorescence from cryoprotectant solutions. It is shown that the fluorescent probes investigated influence the cells motility to different degree.

[The hormone level of the blood from the aorta and the coronary sinus in patients with ischemic heart disease under conditions of preoperative stress].

A total of 156 patients with ischemic heart disease were examined under conditions of preoperative stress in a multiple-modality study performed with the aid of such techniques as echocardiography involving calculation of the myocardial mass index, coronaroventriculography, measurements of the adrenocorticotrophic hormone, hydrocortisone, somatotrophic hormone, triiodothyronine and thyroxin in the blood from the coronary sinus and aorta. A conclusion has been reached that emotional strain influences myocardial hypertrophy. A possible significance of the studied hormonal indices is shown together with features thereof in IHD patients presenting with different degrees of left ventricular hypertrophy.

[Ventricular arrhythmias in patients with ischemic heart disease: a possible role of the autonomous nervous system].

To reveal possible factors that initiate ventricular arrhythmias (VA) in patients with coronary heart disease (CHD), a total of 44 patients with documented coronary artery stenosis were examined. The patients underwent coronary ventriculography, bicycle ergometry, 24-hour Holter monitoring, and examination of the autonomic nervous system (ANS). In 24 patients (Group 1), myocardial ischemic episodes were accompanied by VA.

[The interrelation of the secretory activity of the myocardium with its hypertrophic characteristics in patients with ischemic heart disease].

Evaluation of the concentration of atrial natriuretic peptide, angiotensin P, renin activity in the blood of the coronary sinus and aorta in 18 patients with IHD and hypertrophy of the left ventricle during development of induced ischemia revealed that in left ventricular hypertrophy secretion of atrial natriuretic peptide by the myocardium is reduced. The level of this reduction depends on the kind of hypertrophy. Dilatation of the left ventricle cavity furthers exhaustion of the secretory function of the ischemic myocardium.

[Diastolic filling of the left ventricle in relation to the degree of its hypertrophy in patients with ischemic heart disease].

The features of diastolic filling of the left ventricle (LV) were examined in relation to the nature of its hypertrophy in patients with coronary heart disease (CHD). A total of 110 male patients with CHD concurrent with essential hypertension or without it who underwent contrast ventriculography in order to determine LV diastolic stiffness. Radionuclide ventriculography was performed in 49 patients at rest and during exercise.