[Hormone induced electrokinetic properties of the isolated nuclei of hepatocytes].

It has been investigated the influence of hydrocortisone and estradiol on conforming objects for revealing the correlation between the electrokinetic potential and the physiological state of nuclear membrane. It has been shown that the induction by different hormones leads to many-direct differences in potential. On the basis of obtained results we conclude that the electrokinetic potential may carry an information about functional condition of nuclear membrane and can be a convenient instrument for indirect determination of its physiological state.

[A DNA study of rat liver oligonucleosomes enriched by transcriptionally active genes during induction due to the administration of an amino acid mixture].

A highly active fraction of rat liver oligonucleosome DNA has been isolated and studied by means of thermal denaturation after induction by amino acid mixture or hydrocortisone. A considerable redistribution of DNA content has been shown in sucrose gradient fractions during these forms of induction. The changes are revealed in melting temperature, differential melting profile of DNA, isolated from actively transcribed chromatine fractions.

[Study of the changes in rat liver chromatin after hydrocortisone administration].

After hydrocortisone administration some properties of rat liver chromatin were studied by means of melting technique and circular dichroism. Hydrocortisone caused a more than 2.5-fold increase in amount of the chromatin active fraction.