Comparative Analysis of Active LTR Retrotransposons in Sunflower ( L.): From Extrachromosomal Circular DNA Detection to Protein Structure Prediction.

Plant genomes possess numerous transposable element (TE) insertions that have occurred during evolution. Most TEs are silenced or diverged; therefore, they lose their ability to encode proteins and are transposed in the genome. Knowledge of active plant TEs and TE-encoded proteins essential for transposition and evasion of plant cell transposon silencing mechanisms remains limited.

Quantification of lysosomal labile Zn and monitoring of Zn efflux using a small-molecule-protein hybrid fluorescent probe.

Lysosomal labile Zn levels have been unclear. By targeting a small-molecule fluorescent Zn probe, ZnDA-3H, to lysosomes via VAMP7-Halo, the lysosomal labile Zn concentration was determined to be 1.9 nM in HeLa cells.

Saturation of the compression of two interacting magnetized plasma toroids evidenced in the laboratory.

Interactions between magnetic fields advected by matter play a fundamental role in the Universe at a diverse range of scales. A crucial role these interactions play is in making turbulent fields highly anisotropic, leading to observed ordered fields. These in turn, are important evolutionary factors for all the systems within and around.

Advancing virus-induced gene silencing in sunflower: key factors of VIGS spreading and a novel simple protocol.

Virus-Induced Gene Silencing (VIGS) is a versatile tool in plant science, yet its application to non-model species like sunflower demands extensive optimization due to transformation challenges. In this study, we aimed to elucidate the factors that significantly affect the efficiency of Agrobacterium-VIGS in sunflowers. After testing a number of approaches, we concluded that the seed vacuum technique followed by 6 h of co-cultivation produced the most efficient VIGS results.

Citrulline activates adenosine receptors: New insight into metabolic pathways interaction.

Citrulline is a non-proteinogenic amino acid that forms as by-product in nitric oxide (NO) synthesis from arginine and may act in concert with NO as an independent signaling molecule that involves in the mechanism of vascular smooth muscle vasodilation. In this study we examined the effects of citrulline on pulmonary artery smooth muscles. Experimental design comprised outward potassium currents measurements in enzymatically isolated rat pulmonary artery smooth muscle (PASMc) cells using whole-cell patch clamp technique, isometric contractile force recordings on rat pulmonary artery rings and method of molecular docking simulation.