Model of Ovarian Adenocarcinoma Spheroids for Assessing Photodynamic Cytotoxicity.

Unlabelled: was to compare the relevance of ovarian adenocarcinoma spheroids with that of a monolayer culture for assessing photodynamic effect of the tetrakis(4-benzyloxyphenyl)tetracyanoporphyrazine photosensitizer.

Materials And Methods: The work was performed on SKOV-3 human ovary adenocarcinoma cells grown in a monolayer culture and in the form of tumor spheroids obtained using culture plates with ultra-low attachment. We determined the photoinduced toxicity of porphyrazine on a monolayer culture using the MTT assay; the effect on the spheroids was tested by assessing the dynamics of their growth.