Publications by authors named "A O Burzynski"
Background: The European flounder is readily chosen as an experimental subject and model in physiological and ecotoxicological studies mostly because of its adaptability to laboratory conditions. Many studies utilise a quantitative PCR (qPCR) approach to ascertain the expression of target genes under experimental conditions. Such an approach relies heavily on the selection of reference genes with stable expression.
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- The study focuses on the European flounder, highlighting the lack of comprehensive transcriptome data for this economically important species.
- Researchers generated RNA-Seq data from ten organs of female flounders using Next Generation Sequencing, resulting in 500 million sequencing reads and 61,000 reliable contigs.
- The assembled transcriptome, which demonstrates high completeness according to BUSCOs statistics, can be utilized in various research areas such as biology, aquaculture, and toxicology.
Bivalves are an extraordinary class of animals in which species with a doubly uniparental inheritance (DUI) of mitochondrial DNA have been described. DUI is characterized as a mitochondrial homoplasmy of females and heteroplasmy of male individuals where F-type mitogenomes are passed to the progeny with mother egg cells and divergent M-type mitogenomes are inherited with fathers sperm cells. However, in most cases only male individuals retain divergent mitogenome inherited with spermatozoa.
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- Tools for visualizing genomes are crucial for studying genomic features and interactions, but existing tools fall short for plant mitogenomes due to unique characteristics like non-linear exon arrangements and complex structures.
- The newly developed PMGmap software uses Python to address these limitations by offering detailed visualizations of genes, including noncoding features and chromosomal architecture, effectively scaling genomic regions.
- PMGmap outperformed existing tools in visualizing 405 plant mitogenomes, establishing itself as a valuable resource for researchers in the field, with access available online.
In 2020, the first male-type mitochondrial genome from the clam was published. Apart from the unusual doubly uniparental inheritance of mtDNA, scientists observed a unique (over 4k bp long) extension in the middle of the gene. We have attempted to replicate these data by NGS DNA sequencing and explore further the expression of the long gene.
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