Publications by authors named "A Nobrega"
Objective: To describe indications for and demonstrate the technique to perform a transtracheal aspirate in horses. Transtracheal aspirate is indicated to collect samples for cytology and bacteriologic culture in cases of suspected pneumonia or other lower respiratory tract disease.
Animals: 1 healthy university-owned horse was used for demonstration purposes.
Article Synopsis
- Stress during pregnancy contributes to risks of cardiometabolic disorders and affects fetal liver protein responses, particularly in male offspring.
- A study on Wistar rats showed that prenatal stress elevated maternal corticosterone levels and resulted in lower birth weights, especially in male offspring.
- Male offspring exhibited distinct liver protein expressions related to stress responses, with increased androgen receptor and specific phosphorylation changes, while female offspring showed minimal hepatic impact from prenatal stress.
Coagulopathy is common in equine critical illness, with its early recognition being crucial for patient management and prognosis. In vitro viscoelastic (VE) hypercoagulability with decreased RCM/PCV has been demonstrated in dogs but not horses. Our objective was to evaluate the effects of acepromazine-induced (0.
View Article and Find Full Text PDFMethod validation and determination of pesticides in Mikania glomerta Spreng tincture by direct injection and UPLC-MS/MS analysis.
J Pharm Biomed Anal
January 2025
Over the past two decades, concerns have arisen about the efficacy and safety of medicinal plants, highlighting good agricultural practices and quality control. This study aimed to validate a multi-residue analysis method for detecting 268 pesticides in Mikania glomerata tincture, using direct injection and UPLC-MS/MS, per SANTE guidelines. The validation of the method involved evaluating the linearity of analytical curves in terms of the determination coefficient r2 and residuals (%), as well as the limits of quantification (LOQ), matrix effects, precision (expressed as the coefficient of variation, CV), and accuracy (determined as the recovery percentage).
View Article and Find Full Text PDFBackground And Aims: Prenatal stress may lead to tissue and sex-specific cardiometabolic disorders in the offspring through imbalances in the insulin signaling pathway. Therefore, we aimed to determine the sex-specific adaptations of prenatal stress on the insulin signaling pathway of cardiac and hepatic tissue of adult offspring Wistar rats.
Methods: Wistar pregnant rats were divided into control and stress groups.