Publications by authors named "A Nishihara"

Background: One of the most important and relatively frequent complications of aortic valve replacement is atrioventricular block. It typically occurs by direct injury of the infranodal conduction system due to intra-operative manipulation and persists post-operatively, necessitating permanent pacemaker implantation in many cases.

Case Summary: A 66-year-old man presented to our hospital after experiencing syncope while walking after drinking.

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Anaerobic protists frequently harbour methanogenic archaea, which apparently contribute to the hosts' fermentative metabolism by consuming excess H. However, the ecological properties of endosymbiotic methanogens remain elusive in many cases. Here we investigated the ecology and genome of the endosymbiotic methanogen of the protists in the hindgut of the termite .

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  • The study explores how life utilizing light energy changed Earth's biology and carbon dynamics, highlighting the importance of photosynthesis in shaping today's biosphere.
  • It uses a comprehensive phylogenetic analysis of over 10,000 bacterial genomes to identify the evolutionary connections between bacteria, light metabolism, and carbon fixation.
  • The findings reveal that all current light-metabolizing organisms trace back to a common ancestor, an ancient non-oxygen-producing phototroph, and outline the evolution of light metabolism leading to the rise of oxygen-generating organisms and Cyanobacteria.
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  • The study investigates the occurrence and impact of second primary malignancies (SPMs) in patients who underwent treatment for superficial esophageal squamous cell carcinoma (ESCC).
  • Out of 187 patients monitored over nearly 8 years, there was a 92.6% 5-year survival rate, but 14 patients died from SPMs, particularly from lung cancer, which had a high mortality rate.
  • The research highlights the need for comprehensive screening methods, including both esophagogastroduodenoscopy (EGD) and computed tomography, especially for patients showing certain risk factors like multiple Lugol-voiding lesions and metachronous ESCC.
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We herein propose a fast and easy DNA and RNA co-extraction method for environmental microbial samples. It combines bead beating and phenol-chloroform phase separation followed by the separation and purification of DNA and RNA using the Qiagen AllPrep DNA/RNA mini kit. With a handling time of ~3 h, our method simultaneously extracted high-quality DNA (peak size >10-15‍ ‍kb) and RNA (RNA integrity number >6) from lake bacterioplankton filtered samples.

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