Publications by authors named "A Nemashkalo"
Article Synopsis
- Morphogens are crucial signaling molecules that provide positional information and determine cell fates during embryonic development.
- Using a human gastruloid model, researchers visualized the behavior of the morphogen Nodal, revealing that its influence is limited to neighboring cells and spread through a relay mechanism.
- The study also highlights the role of Lefty, a Nodal inhibitor, which while capable of long-range diffusion, functions locally to regulate the timing of Nodal spread and mesoderm differentiation.
Living cells rely on numerous protein-protein, RNA-protein and DNA-protein interactions for processes such as gene expression, biomolecular assembly, protein and RNA degradation. Single-molecule microscopy and spectroscopy are ideal tools for real-time observation and quantification of nucleic acids-protein and protein-protein interactions. One of the major drawbacks of conventional single-molecule imaging methods is low throughput.
View Article and Find Full Text PDFParacrine signals maintain developmental states and create cell fate patterns and influence differentiation outcomes in human embryonic stem cells (hESCs) Systematic investigation of morphogen signaling is hampered by the difficulty of disentangling endogenous signaling from experimentally applied ligands. Here, we grow hESCs in micropatterned colonies of 1-8 cells ('µColonies') to quantitatively investigate paracrine signaling and the response to external stimuli. We examine BMP4-mediated differentiation in µColonies and standard culture conditions and find that in µColonies, above a threshold concentration, BMP4 gives rise to only a single cell fate, contrary to its role as a morphogen in other developmental systems.
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