Quantification of Filovirus Soluble Glycoprotein Using a Sandwich ELISA.

Sandwich enzyme-linked immunosorbent assays (ELISAs), which quantify antigens captured between two layers of antibodies, are sensitive and fundamental tools used to diagnose diseases, evaluate the effects of countermeasures, and quantify target proteins. Many filoviruses use transcriptional editing to express three different glycoproteins (GPs) from the GP gene. The main product transcribed from the GP gene, the soluble glycoprotein (sGP), is detected at high levels in the blood during the acute phase of infection and correlates with viremia, making it an attractive target for molecular diagnostics.

Marburg virus exploits the Rab11-mediated endocytic pathway in viral-particle production.

Filoviruses produce viral particles with characteristic filamentous morphology. The major viral matrix protein, VP40, is trafficked to the plasma membrane and promotes viral particle formation and subsequent viral egress. In the present study, we assessed the role of the small GTPase Rab11-mediated endocytic pathway in Marburg virus (MARV) particle formation and budding.

Micro-second time-resolved X-ray single-molecule internal motions of SARS-CoV-2 spike variants.

Single-molecule intramolecular dynamics were successfully measured for three variants of SARS-CoV-2 spike protein, alpha: B.1.1.