Registration of activity of a single molecule of horseradish peroxidase using a detector based on a solid-state nanopore.

This work demonstrates the use of a solid-state nanopore detector to monitor the activity of a single molecule of a model enzyme, horseradish peroxidase (HRP). This detector includes a measuring cell, which is divided into cis- and trans- chambers by a silicon nitride chip (SiN structure) with a nanopore of 5 nm in diameter. To entrap a single HRP molecule into the nanopore, an electrode had been placed into the cis-chamber; HRP solution was added into this chamber after application of a negative voltage.

No time to relax: Age-dependent infectivity of cercariae in marine coastal ecosystems.

Age dynamics of the ability of cercariae of two digenean species, (Himasthlidae) and (Renicolidae), to infect the second intermediate host (SIH), mussels (), was investigated experimentally. This is the first study of this kind made on cercariae transmitted in the intertidal of the northern seas. The larvae of all tested ages (from 0.

[Quality of life in patients with primary hyperparathyroidism before and at different time-points after surgery and factors associated with its improvement].

Background: For a comprehensive assessment of the effect of surgery in patients with primary hyperparathyroidism (PHPT) it sounds reasonable to evaluate quality of life (QoL) and symptoms in PHPT patients at long-term after parathyroidectomy (PTE). The purpose of this study was to study the quality of life of patients with PHPT before and at different times after PTE and to determine the factors associated with its improvement after surgery.

Materials And Methods: During prospective observational study, patients filled out QoL questionnaires before, 3, 12, 24 months or more after surgery.

Enriched Environment Induces Sex-Specific Changes in the Adult Neurogenesis, Cytokine and miRNA Expression in Rat Hippocampus.

An enriched environment stimulates adult hippocampal plasticity, but the exact cellular and molecular mechanisms are complex, and thus a matter of debate. We studied the behavior and hippocampal neurogenesis in adult male and female Wistar rats that were housed in an enriched environment (EE) for two months. Both EE males and females performed better than control animals in a Barnes maze, meaning that EE enhances spatial memory.