[Photogenerator of trichloroacetic acid--a perspective detritylation agent for microchip oligonucleotide synthesis].

For the purpose of new detritylation agents search for microarray oligonucleotide synthesis we investigated applicability of [4-(4-methoxyphenyl)-2,6-dinitro-phenyl](phenyl)methyl 2,2,2-trichloroacetate for 4,4'-dimethoxytrityl group detritylation during oligonucleotide syntheses generating trichloroacetic acid at radiation by light with a length of wave of 405 nanometers. [4-(4-Methoxyphenyl)-2,6-dinitro-phenyl](phenyl)methyl 2,2,2-trichloroacetate has been successfully used for solid-phase oligonucleotide synthesis of desired oligonucleotides.

[A rare case of thalassemia concomitant with chronic decompensated tonsillitis].

The present paper reports a rare combination of Cooley's disease (thalassemia B) and the decompensated variant of chronic tonsillitis in a 14-year old girl. The patient presented with the severe form of hypochromic anemia and degenerative changes of erythrocytes. She was treated by means of bilateral tonsillectomy associated with the high risk of postoperative hemorrhage.

[A compact microarray for sub-typing of influenza A virus].

A universal oligonucleotide hybridazation microchip 6 x 5 spot (4 x 4 mm) for influenza A virus subtyping was suggested, functioning on a principle one spot--one subtype. This microchip with additional printing quality control is a prototype of a biosensor for detection of influenza A virus and typing of 15 subtypes of hemagglutinin and 9 subtypes of neuraminidase.

[Oligo(2'-O-Methylribonucleotides) and their derivatives: IV. Conjugates of oligo(2'-O-methylribonucleotides) with minor groove binders and intercalators: synthesis, properties and application].

Conjugates of pyrimidine triplex forming 3'-protected oligo(2'-O-methylribonucleotides) with minor groove binders (MGB) and triplex specific intercalator benzoindoloquinoline (BIQ) at 5'-terminus were synthesized. The conjugates formed stable complexes with target dsDNA by simultaneous binding both in its minor and major grooves and BIQ intercalation. The dissociation constants and thermal stability of the conjugate complexes with model dsDNA corresponding to polypurine tract (PPT) of genes nef and pol from HIV proviral genome were determined.