[Effect of ribosomal proteins of respiratory bacteria on adhesive properties of buccal epitheliocytes].

Aim: Study the effect of ribosomal proteins of respiratory bacteria composing the basis of the immune-modulating preparation ribomunil on adhesive properties of buccal epithelium of healthy donors, and carry out comparison of this parameter during use of other bacterial products.

Materials And Methods: Various amounts of bacterial ribosomal proteins, Escherichia coli (serotype O127:B8) and one-day Staphylococcus aureus (strain 5983) culture supernatant were added to "buccal epitheliocytes--candida" system and incubated. Buccal cells were washed after the incubation from non-bound candida and differentiated microscopically by the amount of cells with various levels of candida adhesion.

[Neutrophils and bacterial biofilms: dialectics of relationship].

The review is dedicated to the problem of interaction of human neutrophils with bacterial biofilms that complicate the course of infectious process. Neutrophils being the most important effectors of innate immunity may attack bacterial biofilms causing their rejections and damage of biofilm microbes. Mechanisms of neutrophil-dependent destruction of biofilms are analyzed in the review.

Vesicle formation as a result of interaction between polymorphonuclear neutrophils and Staphylococcus aureus biofilm.

Staphylococcus aureus, a major opportunistic pathogen, is a leading cause of biofilm-related infections in clinical practice. Staphylococcal biofilms are highly resistant to antibacterial medicines and immune effector cells. The main result of our work is the discovery of nano-vesicles in the supernatant of the human neutrophil-S.

[Pseudomonas aeruginosa: characteristics of biofilm process].

Definition of the biofilm process as one of the types of intercellular bacterial communications is presented. The modern data concerning the structure of the Pseudomonas aeruginosa biofilm matrix and genetic mechanisms necessary for its production are described. Active and passive rejections of biofilm bacteria, which are the basis of bacterial spreading to new surfaces, are discussed.

[Interspecies interaction of bacteria and the formation of mixed (polymicrobial) biofilm].

Natural biofilms rarely exist as monocultures. Usually they are formed from various microorganism species that interact with each other, have shared metabolites, strengthen the attachment of each other to the support substrate, provide expression of "foreign" genes etc. Material on factors and mechanisms that determine the formation of mixed (polymicrobial) biofilms is analyzed in the review.