Characterization and implementation of the MarathonRT template-switching reaction to expand the capabilities of RNA-seq.

End-to-end RNA-sequencing methods that capture 5'-sequence content without cumbersome library manipulations are of great interest, particularly for analysis of long RNAs. While template-switching methods have been developed for RNA sequencing by distributive short-read RTs, such as the MMLV RTs used in SMART-Seq methods, they have not been adapted to leverage the power of ultraprocessive RTs, such as those derived from group II introns. To facilitate this transition, we dissected the individual processes that guide the enzymatic specificity and efficiency of the multistep template-switching reaction carried out by RTs, in this case, by MarathonRT.

Truncated hemoglobin 2 modulates phosphorus deficiency response by controlling of gene expression in nitric oxide-dependent pathway in Chlamydomonas reinhardtii.

Truncated hemoglobin 2 is involved in fine-tuning of PSR1-regulated gene expression during phosphorus deprivation. Truncated hemoglobins form a large family found in all domains of life. However, a majority of physiological functions of these proteins remain to be elucidated.

Truncated Hemoglobins 1 and 2 Are Implicated in the Modulation of Phosphorus Deficiency-Induced Nitric Oxide Levels in .

Truncated hemoglobins (trHbs) form a widely distributed family of proteins found in archaea, bacteria, and eukaryotes. Accumulating evidence suggests that trHbs may be implicated in functions other than oxygen delivery, but these roles are largely unknown. Characterization of the conditions that affect trHb expression and investigation of their regulatory mechanisms will provide a framework for elucidating the functions of these globins.

The regional elastic properties analysis of myocardium based on echocardiographic 3-D reconstruction of the left ventricle.

The present study evaluates the myocardium regional elastic properties on the basis of relative thickness change (DeltaHWT) in the left ventricular (LV) wall during the diastolic filling phase. Two-dimensional (2-D) LV long-axis images were obtained with a Powervision-380 (Toshiba) transesophageal echocardiographic imager. Three-dimensional (3-D) reconstruction of the LV was carried out by rotation of the transducer in calibrated steps.

'Minimum average risk' as a new peak-detection algorithm applied to myofibrillar dynamics.

We present a new peak-detection algorithm based on the method of 'minimum average risk' proposed by Kolmogorov and developed for signal processing in various fields. In this method, translations of features within a signal scan are quantified by minimizing the integrated pointwise product of each scan relative to the first derivative of the immediately previous scan. We have adapted this method for use in a new algorithm to monitor dynamic changes of sarcomere length in single myofibrillar sarcomeres of striated muscles, but the algorithm can also be used more generally for peak localization.