Biophysics of Evolution of Intellectual Systems.

The first work on noogenesis as evolution of intellect was published 150 years ago. However, it was not until the 21st century that quantitation became possible for certain parameters that contribute to the understanding of the evolution of intellectual systems in natural sciences, the progress being due to basic achievements in physics, biology, medicine, and interdisciplinary fields. Analyses of the parameters of intellectual systems, patterns of their emergence and evolution, distinctive features, and the constants and limits of their structures and functions made it possible to measure and compare the capacity of communications (~100 to 300 million m/s), to quantify the number of components in intellectual systems (10-100 billion components), and to calculate the number of successful links responsible for cooperation (from 150 to 1 trillion links).

[Bases of information hygiene: concepts and problems of innovations].

The rising flow of information affects the health and affords ground for strengthening measures of hygienists. There is considered the place of information hygiene in preventive medicine. There are discussed physical and hygienic aspects of measurement and evaluation of information, there are considered some of their negative consequences.

Isolation, purification, and characterization of catalase from the methylotrophic yeast Pichia pastoris.

Catalase (CATpp) with molecular weight 223 kD was isolated from the methylotrophic yeast Pichia pastoris and purified 90-fold by ion-exchange chromatography and gel filtration. Quantitative parameters of absorption and CD spectra of CATpp solutions and of its membrane-concentrated form (CATpp-conc) were studied. Rates of H2O2 decomposition and kinetic characteristics Km and kcat of CATpp and CATpp-conc were determined in 10 mM phosphate buffer (pH 7.

Initiation and inhibition of free radical processes in H2O2-metmyoglobin (methemoglobin)-2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) systems.

Rates of free radical initiation were determined at 20 degrees C in 10 mM phosphate buffer (pH 7.4) in the systems metmyoglobin (methemoglobin)-H2O2 using 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as the diammonium salt (ABTS). The catalytic activity of MetMb was 2-3-fold higher than that of MetHb.

Effect of gallic acid polydisulfide on activity and stability of catalase in various media.

The effect of the active bioantioxidant polydisulfide of gallic acid (PDSG) on the catalytic activity and operational and thermal stability of catalase was studied in three media: distilled water (pH approximately 5.6), phosphate buffer, pH 7.4, and reversed micelles of Aerosol OT (AOT) in heptane of varied hydration degree w0.