[Experience in the clinical-experimental use of ointments in combination with hyperbaric oxygenation].

The results of performance of 317 experimental and 1986 clinical procedures of hyperbaric oxygenation with the use of ointments and oils are summarized. The authors recommend to use the suggested technique for the treatment of patients with sluggish wounds, trophic ulcers and burn wounds.

[The cryopreservation of isolated liver cells].

Recent literature data on cryopreservation of isolated hepatocytes are summarized. The hepatocytes are considered to constitute a promising system for investigation of liver metabolic peculiarities as well as for elucidating mechanisms of low temperature effects on biological systems.

[A non-enzymatic method of preparing isolated hepatocytes: assessment of their morpho-functional activity].

A method for isolating hepatocytes using the two-step liver perfusion by chelate-containing solutions is described. The hepatocytes obtained are highly inactive for the trypan blue staining test, (not less than 90%), ATP content, the rate of endogenic respiration and its stimulation by succinate, a degree of xenobiotic oxidation.

[Functional characteristics of rat hepatocytes isolated by a nonenzymatic method and incubated in vitro].

A study was made of the effect of normothermic incubation on the metabolic state of parenchymal liver cells, isolated by the nonenzymatic method. In the process of a 2 hour incubation in the nutritious sodium-containing medium, a high level of the energy supply system is maintained, determined by the contents of adenine nucleotides, the rate of endogenous respiration and by its stimulation by succinate.