Publications by authors named "A Mouithys-Mickalad"
Article Synopsis
- - Laminitis in horses causes severe pain and can lead to euthanasia, prompting research for better treatments through an in vitro model that tests cell responses to stressful conditions.
- - The study found that during reoxygenation, keratinocytes (skin cells) show reduced metabolism, indicating cellular stress, but introducing muscle-derived stem cells helped restore their function.
- - Additionally, exposure to activated neutrophils increased cell activity related to inflammation, which stem cells could help regulate, suggesting their potential use in treating laminitis through cell therapy.
Article Synopsis
- - The study explores the use of equine platelet lysate (ePL) as a replacement for fetal bovine serum (FBS) in growing muscle-derived mesenchymal stromal cells (mdMSCs) for regenerative medicine, addressing ethical and practical issues associated with FBS.
- - Two innovative 2D and 3D culture models were tested, with the 3D model yielding a higher total cell count than the 2D model, while both models confirmed the multipotent capabilities of mdMSCs through differentiation assays.
- - The mdMSCs showed promising immunomodulatory properties, significantly inhibiting myeloperoxidase (MPO) and reactive oxygen species (ROS) activity, especially in the
The main causes of mortality in horses are the gastrointestinal pathologies associated with septic shock. Stem cells have shown, through systemic injection, a capacity to decrease inflammation and to regenerate injured tissue faster. Nevertheless, to achieve this rapid and total regeneration, systemic injections of 1 to 2 million cells per kilogram of body weight must be considered.
View Article and Find Full Text PDFLaminitis is a pathology of the equine digit ultimately leading to a failure of the dermo-epidermal interface. Neutrophil activation is recognized as a major factor in SIRS-associated laminitis and has recently been described in induced endocrinopathic laminitis evidenced by the presence of myeloperoxidase (MPO). Neutrophil extracellular traps (NET) are released with neutrophil activation.
View Article and Find Full Text PDFThere is a growing interest in the use of natural compounds to tackle inflammatory diseases and cancers. However, most of them face the bioavailability and solubility challenges to reaching cellular compartments and exert their potential biological effects. Polyphenols belong to that class of molecules, and numerous efforts have been made to improve and overcome these problems.
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