Chitosan based hydrogel for iron (III) chelation in biological conditions.

In this study, a chitosan derivative with strong iron (III) chelating capabilities was developed by grafting the Deferoxamine (DFO) chelator to achieve a substitution degree of 3.8 ± 0.2 %.

Bio-functionalized hydrogel patches of chitosan for the functional recovery of infarcted myocardial tissue.

The aim of this work was to assess the potential benefits of the enrichment of a chitosan hydrogel patch with secretome and its epicardial implantation in a murine model of chronic ischemia, focusing on the potential to restore the functional capacity of the heart. Thus, a hydrogel with a final polymer concentration of 3 % was prepared from chitosan with an acetylation degree of 24 % and then bio-functionalized with a secretome produced by mesenchymal stromal cells. The identification of proteins in the secretomes showed the presence of several proteins known to have beneficial effects on cardiac muscle repair.

Nanoassemblies of Chitosan-Based Polyelectrolyte Complexes as Nucleic Acid Delivery Systems.

Nucleic acid delivery requires vectorization for protection from nucleases, preventing clearance by the reticuloendothelial system, and targeting to allow cellular uptake. Nanovectors meeting the above specifications should be safe for the patient, simple to manufacture, and display long-term stability. Our nanovectors were obtained via the green process of polyelectrolyte complexation, carried out at 25 °C in water at a low shear rate using chitosan (a polycationic biocompatible polysaccharide of specific molar mass and acetylation degree) and dextran sulfate as a polyanionic biocompatible polysaccharide.

Stretchable hydrogels of chitosan/hyaluronic acid induced by polyelectrolyte complexation around neutral pH.

In this work, we propose the formation of stretchable hydrogels at neutral pH from the physical crosslinking of chitosan (CS) and hyaluronic acid (HA) by polyelectrolyte complexation. A mixture of CS (M ≈ 600 kg/mol, degree of acetylation ≈ 50 %) solution and HA (M ≈ 77 kg/mol) solution was prepared with an excess of salts screening the electrostatic interactions CS/HA. In a controlled manner, the polyelectrolyte complexation was induced through the progressive dialysis of the salted polymer mixture against a sodium acetate solution (AcONa, 0.