Publications by authors named "A Middleton"
For many years, a method that allowed systemic toxicity safety assessments to be conducted without generating new animal test data, seemed out of reach. However, several different research groups and regulatory authorities are beginning to use a variety of in silico, in chemico and in vitro techniques to inform safety decisions. To manage this transition to animal-free safety assessments responsibly, it is important to ensure that the level of protection offered by a safety assessment based on new approach methodologies (NAMs), is at least as high as that provided by a safety assessment based on traditional animal studies.
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- General RNA chaperones are proteins that help RNA molecules fold correctly and avoid misfolding, which is crucial for bacteria, especially with large and complex RNA structures.
- Researchers adapted a technique to screen for factors affecting RNA folding and identified eight proteins that influence the folding of a specific RNA ribozyme when deleted.
- Among these, YagL and PepA were found to bind RNA and assist in the folding process, with YagL being especially effective in promoting correct refolding of misfolded RNA, thanks to a specific protein domain responsible for its activity.
The primary purpose of the original NAPCHD project was to develop a national Strategic Document of Diabetes Care for Care Homes which has now been completed and well received as a worthwhile, sustainable, and effective guidance for delivering quality diabetes care in the UK. A Working Group of NAPCHD was established to produce a Position Statement on type 1 diabetes in care homes since this area was recommended as a topic to further develop. There are currently limited data on the prevalence and clinical outcomes associated with type 1 diabetes in care homes and management policies have been non-existent in the UK.
View Article and Find Full Text PDFThe ubiquitin-conjugating E2 enzymes play a central role in ubiquitin transfer. Disruptions to the ubiquitin system are implicated in multiple diseases, and as a result, molecules that modulate the activity of the ubiquitin system are of interest. E2 enzyme function relies on interactions with partner proteins, and the disruption of these is an effective way to modulate activity.
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- The study investigates whether living in rural areas affects mortality rates in patients with acute myocardial infarction (AMI) and heart failure (HF), which may impact healthcare access and outcomes.
- A meta-analysis of 37 studies was conducted, encompassing over 21 million AMI patients and nearly 18 million HF patients, to compare rural versus urban patient mortality, focusing on all-cause mortality.
- Results indicate that although rural AMI patients are slightly older and have a higher proportion of females, the analysis aims to provide insights into mortality differences and the factors that may contribute to any observed disparities between rural and urban populations.