Publications by authors named "A Mdunyelwa"
Article Synopsis
- The Seegene Allplex™ RV Master (RVM) assay is a multiplex RT-PCR tool designed to detect eight viral respiratory pathogens from various specimens, including those collected via nasopharyngeal swabs.
- The study aimed to assess the diagnostic effectiveness of the RVM assay by calculating various performance metrics like sensitivity, specificity, and predictive values compared to established diagnostic methods.
- A total of 249 positive and numerous negative respiratory specimens were analyzed, ensuring comprehensive evaluation of the RVM assay's reliability and its performance across different extraction workflows.
The rapid emergence and spread of numerous severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants across the globe underscores the crucial need for continuous SARS-CoV-2 surveillance to ensure that potentially more pathogenic variants are detected early and contained. Whole genome sequencing (WGS) is currently the gold standard for COVID-19 surveillance; however, it remains cost-prohibitive and requires specialized technical skills. To increase surveillance capacity, especially in resource-scarce settings, supplementary methods that are cost- and time-effective are needed.
View Article and Find Full Text PDFWe describe a case of prolonged SARS-CoV-2 RNA shedding in an HIV-negative 21-year-old man recovering from abdominal and thoracic trauma. Nasopharyngeal (NP) swabs collected at 12 time points over a 95-day span all tested positive for SARS-CoV-2 by reverse transcription polymerase chain reaction (RT-PCR). Genotyping revealed canonical beta-variant E484K and N501Y mutations at earlier time points.
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