Cleavage Under Targets and Release Using Nuclease (CUT&RUN) of Epigenetic Regulators.

Chromatin immunoprecipitation followed by sequencing (ChIP-Seq) allows for the identification of genomic targeting of DNA-binding proteins. Cleavage Under Targets and Release Using Nuclease (CUT&RUN) modifies this process by including a nuclease to digest DNA around a protein of interest. The result is a higher signal-to-noise ratio and decreased required starting material.

Direct observation of twisted stacking domains in the van der Waals magnet CrI.

Van der Waals (vdW) stacking is a powerful technique to achieve desired properties in condensed matter systems through layer-by-layer crystal engineering. A remarkable example is the control over the twist angle between artificially-stacked vdW crystals, enabling the realization of unconventional phenomena in moiré structures ranging from superconductivity to strongly correlated magnetism. Here, we report the appearance of unusual 120° twisted faults in vdW magnet CrI crystals.

Research culture in biomedicine: what we learned, and what we would like to do about it.

The significant stressors that affect the biomedical research community have the potential to negatively impact the conduct of science. Here we report on work done at Harvard Medical School to identify areas for improvement in research rigor, reproducibility, and responsibility in pursuit of continued research excellence.

Outcomes of stereoelectroencephalography following failed epilepsy surgery in children.

Introduction: Stereoelectroencephalography (SEEG) is valuable for delineating the seizure onset zone (SOZ) in pharmacoresistant epilepsy when non-invasive presurgical techniques are inconclusive. Secondary epilepsy surgery after initial failure is challenging and there is limited research on SEEG following failed epilepsy surgery in children.

Objective: The objective of this manuscript is to present the outcomes of children who underwent SEEG after failed epilepsy surgery.