Lineage Recording in Human Brain Organoids with iTracer.

Induced pluripotent stem cell (iPSC)-derived organoids provide models to study human organ development. Single-cell transcriptomics enables highly resolved descriptions of cell states within these systems; however, approaches are needed to directly determine the lineage relationship between cells. Here we provide a detailed protocol (Fig.

The Need for Early Engagement with Interested Groups on Advanced Biopreservation.

Research on advanced biopreservation - technologies that include, for example, partial freezing, supercooling, and vitrification with nanoparticle infusion and laser rewarming - is proceeding at a rapid pace, potentially affecting many areas of medicine and the life sciences, food, agriculture, and environmental conservation. Given the breadth and depth of its medical, scientific, and corresponding social impacts, advanced biopreservation is poised to emerge as a disruptive technology with real benefits, but also ethical challenges and risks. Early engagement with potentially affected groups can help navigate possible societal barriers to adoption of this new technology and help ensure that emerging capabilities align with the needs, desires, and expectations of a broad range of interested parties.

Building Governance & Coordination Across Applications.

Advanced biopreservation technologies using subzero approaches such as supercooling, partial freezing, and vitrification with reanimating techniques including nanoparticle infusion and laser rewarming are rapidly emerging as technologies with potential to radically disrupt biomedicine, research, aquaculture, and conservation. These technologies could pause biological time and facilitate large-scale banking of biomedical products including organs, tissues, and cell therapies.

Successfully Bridging Innovation and Application: Exploring the Utility of a Risk Innovation Approach in the NSF Engineering Research Center for Advanced Biopreservation Technologies (ATP-Bio).

This exploratory study set out to pilot use of a Risk Innovation approach to support the development of advanced biopreservation technologies, and the societally beneficial development of advanced technologies more broadly. This is the first study to apply the Risk Innovation approach - which has previously been used to help individual organizations clarify areas of value and threats - to multiple entities involved in developing an emerging technology.