Publications by authors named "A Matveeva"
tRNA-derived stress-induced RNAs (tiRNAs) are a new class of small non-coding RNA that have emerged as important regulators of cellular stress responses. tiRNAs are derived from specific tRNA cleavage by the stress-induced ribonuclease angiogenin (ANG). Loss-of-function mutations in the ANG gene are linked to amyotrophic lateral sclerosis (ALS), and elevated levels of specific tiRNAs were recently identified in ALS patient serum samples.
View Article and Find Full Text PDFColorectal cancer (CRC) is one of the most frequently occurring cancers, but prognostic biomarkers identifying patients at risk of recurrence are still lacking. In this study, we aimed to investigate in more detail the spatial relationship between intratumoural T cells, cancer cells, and cancer cell hallmarks as prognostic biomarkers in stage III colorectal cancer patients. We conducted multiplexed imaging of 56 protein markers at single-cell resolution on resected fixed tissue from stage III CRC patients who received adjuvant 5-fluorouracil (5FU)-based chemotherapy.
View Article and Find Full Text PDFCurrently, the CRISPR-Cas9 system serves as a prevalent tool for genome editing and gene expression regulation. Its therapeutic application is limited by off-target effects that can affect genomic integrity through nonspecific, undesirable changes in the genome. Various strategies have been explored to mitigate the off-target effects.
View Article and Find Full Text PDFArticle Synopsis
- mRNA-based therapies have gained popularity over the last decade, particularly highlighted by their role in mass COVID-19 vaccination, leading to further research into antiviral and anti-cancer vaccines and genetic treatments.
- Lipid nanoparticles (LNPs) are crucial for mRNA delivery, and adaptable LNP systems are needed to better control how mRNA is taken up and expressed in target cells.
- New cationic lipid formulations (2X3 and 2X7) have shown effective mRNA delivery in lab cells and live mice, demonstrating prolonged gene activity and localized expression in muscles, pointing to their potential for long-term therapeutic applications.
Article Synopsis
- Researchers aimed to develop a cellular model to study the effects of tumor necrosis factor (TNF) depending on the presence or absence of TNFR1 and TNFR2 receptors in cell lines.
- They created TNFR1 knockout versions of ZR-75/1 and K-562 cell lines to analyze how this absence affects receptor distribution, cell cycle, cell death, and gene expression in response to TNF.
- Findings showed that removing TNFR1 led to changes in TNFR2 distribution, influencing sensitivity to TNF and altering cell proliferation and death patterns in different ways across the two cell lines.