Immune recognition of lysyl-tRNA synthetase and isoleucyl-tRNA synthetase by anti-OJ antibody-positive sera.

Objective: Anti-aminoacyl-tRNA synthetase (anti-ARS) antibodies are useful for identifying a clinical subset of patients with idiopathic inflammatory myopathies (IIMs). Anti-OJ antibodies, which recognize multi-enzyme synthetase complexes including isoleucyl-tRNA synthetase (IARS) and lysyl-tRNA synthetase (KARS), are among the anti-ARS antibodies. Although testing antibodies to other ARSs have been used clinically, no validated immunoassays for detecting anti-OJ antibodies are available.

In vitro and in vivo activity of penicillinase inhibitor KA-107 against Staphylococcus aureus FS-1277.

The activities of penicillinase inhibitor, KA-107 extracted from culture filtrates of Streptomyces gedanensis ATCC 4880, were studied by using penicillin-resistant S. aureus FS-1277 and by means of in vitro and in vivo tests.