Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus.

In eukaryotes the 40S and 60S ribosomal subunits are assembled in the nucleolus, but there appear to be mechanisms preventing mRNA binding, 80S formation, and initiation of translation in the nucleus. To visualize association between ribosomal subunits, we tagged pairs of Drosophila ribosomal proteins (RPs) located in different subunits with mutually complementing halves of fluorescent proteins. Pairs of tagged RPs expected to interact, or be adjacent in the 80S structure, showed strong fluorescence, while pairs that were not in close proximity did not.

Conceptual problems of colon pathologies of surgical interest in the elderly.

Case reports of 151 patients above the age of 70 years, treated with colon diseases of surgical interest, have been compared to those of 220 patients treated with similar diagnoses, but in younger ages. The case reports were evaluated on the basis of the available literary data, and interpreted from the points of view of the geriatric pathology. It is concluded that a correct interpretation of surgical pathologies of the colon in the elderly cannot disregard the knowledge of the age-dependent involutive processes concerning particularly this part of the digestive tract, and also other organs and systems in global sense.