Evaluation of genetic variability of wild hops (Humulus lupulus L.) in Canada and the Caucasus region by chemical and molecular methods.

Wild hops (Humulus lupulus L.) are potential new germplasms to expand the variability of genetic resources for hop breeding. We evaluated Canadian (62 plants) and Caucasian (58 plants) wild hops by their chemical characteristics and with molecular genetic analyses using sequence-tagged site and simple sequence repeat markers, in comparison with European (104 plants) and North American (27 plants) wild hops.

[The role of thyroid hormones in the regulation of electric stability of erythrocyte membranes in rats].

A method of electric breakdown was used for a study of electric stability (durability) of the membranes of erythrocytes isolated from the blood of normal rats and rats with hypo-, hyperthyrosis and thyrotoxicosis. Erythrocytic membrane electric durability was shown to depend on the hormonal status. In particular, an increase in the potential of breakdown of erythrocytes of hyperthyroid rats was shown in comparison with the characteristics of erythrocytes of the other studied populations.

[Effect of thyroid hormones on the physico-chemical properties of lipids in the liver mitochondria of rats].

The administration of thyroxine to rats (300 micrograms per 100 g of body mass intraperitoneally) resulted in a decrease in the content of cardiolipin, cholesterol and an increase in lysophosphatidylethanolamine in rat mitochondria as compared to normal in 48 hours. During the registration of spin-labeled mitochondrial lipids using the EPR method thyroid-related changes in the regulation parameter on Arrenius' charts were detected suggesting the regulation of the physical state of mitochondrial lipids by thyroid hormones. It was assumed that the observed hormone-related modifications of the physicochemical state of mitochondrial lipids illustrated the membranotropic mechanism of the regulation of mitochondrial functions by thyroid hormones.

[Characteristics of the levels of various nucleic acid precursors in the tissues of rabbits with various thyroid conditions].

The content of the precursors and metabolites of nucleic acids in the brain and liver of normal rabbits, rabbits with hypothyrosis (thyroidectomy) and hyperthyrosis (100-150 Mg of L-thyroxine per 1 kg of body mass daily for 10-12 days) was determined by liquid chromatography with a high resolution. A decrease in the content of deoxyguanosine, thymine, hypoxanthine, cytosine, thymidine and an increase in the content of inosine, adenine and adenosine in the liver of animals with hypothyrosis were shown. In the brain of these rabbits the level of cytidine was lowered, and the levels of deoxyguanosine, thymine, inosine, hypoxanthine, adenine and uracil were on an increase.

[Role of mitochondrial peroxidation of lipids in their hydrolysis by endogenous phospholipase A2].

The analysis of lipids (18 compounds all in all) obtained from mitochondria and incubated for two hours was carried out. It has been shown that hydrolysis of individual phospholipids by endogenous phospholipase of these organelles depends on the intensity of lipid peroxidation (LPO). Thus, with the low level of this process the content of phosphatidylethanolamine, cardiolipin and phosphatidylcholine decreased by 25%, 33% and 18%, respectively of the initial level.