Systematic comparison of quantity and quality of RNA recovered with commercial FFPE tissue extraction kits.

Background: FFPE tissue samples are commonly used in biomedical research and are a valuable source for next-generation sequencing in oncology, however, extracting RNA from these samples can be difficult the quantity and quality achieved can impact the downstream analysis. This study compared the effectiveness of seven different commercially available RNA extraction kits specifically designed for use with FFPE samples in terms of the quantity and quality of RNA recovered.

Methods: This study used 9 samples of FFPE tissue from three different types of tissue (Tonsil, Appendix and lymph node of B-cell lymphoma) to evaluate RNA extraction methods.

First report of warble fly infestation in markhor, .

In December 2023, 35 third-instar larvae were collected from subcutaneous granulomatous cysts in a free-ranging markhor () hunted in the Dashtijum region of the Khazratishoh mountain range, Tajikistan. The larvae were identified as those of (Diptera: Oestridae: Hypodermatinae). To the best of our knowledge, this is the first report of an oestrid fly infestation in this host species.

Evaluation of energy efficiency actions in Mexican aqueducts with an approach on their performance over time. Case study: Northwest Mexico aqueduct.

Transporting bulk water from cities where it is scarce requires significant amounts of energy. It is estimated that 7 % of the world's energy production is consumed by pumping systems. Most energy efficiency actions are not applicable to systems of bulk water distribution.

ATM and IRAK1 orchestrate two distinct mechanisms of NF-κB activation in response to DNA damage.

DNA damage in cells induces the expression of inflammatory genes. However, the mechanism by which cells initiate an innate immune response in the presence of DNA lesions blocking transcription remains unknown. Here we find that genotoxic stresses lead to an acute activation of the transcription factor NF-κB through two distinct pathways, each triggered by different types of DNA lesions and coordinated by either ataxia-telangiectasia mutated (ATM) or IRAK1 kinases.

Economic preferences and cognitive abilities among teenagers in Spain.

This dataset originates from TeensLab, a consortium of Spanish Universities dedicated to behavioral research involving Spanish teenagers. The dataset contains data from 33 distinct educational institutions across Spain, accounting for a total of 5,890 students aged 10 to 23 (M = 14.10, SD = 1.