RAPD and phytochemical analysis of Thymus moroderi plantlets after cryopreservation.

Cryopreservation is at present the most reliable strategy to preserve plant germplasm. When aromatic plants are the object of conservation it is necessary to assess not only the genetic but also the phytochemical stability to ensure that plant material maintains its qualities after storage. In this work we present molecular and phytochemical stability data related to a previously described vitrification-based cryopreservation protocol for Thymus moroderi Pau ex Martínez.

Comparison of vitrification and encapsulation-dehydration for cryopreservation of Thymus moroderi shoot tips.

Vitrification and encapsulation-dehydration were tested for cryopreservation of Thymus moroderi Pau ex Martínez (Labiatae), an endemic plant from south-eastern Spain. For vitrification, shoot tips were loaded in a solution containing 0.4 M sucrose + 2 M glycerol for 20 min at room temperature, dehydrated in PVS2 solution for 0-105 min at 0 degree C, then immersed in liquid nitrogen (LN) for at least 1 day and rapidly rewarmed.

Cryopreservation of Thymus moroderi by droplet vitrification.

Thymus moroderi Pau ex Martinez (Labiatae) was successfully cryopreserved using the droplet vitrification method. After 20 min in loading solution at room temperature, shoot tips were dehydrated with PVS2 at 0 degree C for 30 min and immersed into LN. For thawing, shoot-tips were transferred into recovery solution for 15 min.