Peptide-Oligonucleotide Conjugation: Chemistry and Therapeutic Applications.

Oligonucleotides have been identified as powerful therapeutics for treating genetic disorders and diseases related to epigenetic factors such as metabolic and immunological dysfunctions. However, they face certain obstacles in terms of limited delivery to tissues and poor cellular uptake due to their large size and often highly charged nature. Peptide-oligonucleotide conjugation is an extensively utilized approach for addressing the challenges associated with oligonucleotide-based therapeutics by improving their delivery, cellular uptake and bioavailability, consequently enhancing their overall therapeutic efficiency.

A Simplified Calibration Procedure for DEM Simulations of Granular Material Flow.

The discrete element method (DEM) has emerged as an essential computational tool in geotechnical engineering for the simulation of granular materials, offering significant advantages over traditional continuum-based methods such as the finite element method (FEM) and the finite difference method (FDM). The DEM's ability to model particle-level interactions, including contact forces, rotations, and particle breakage, allows for a more precise understanding of granular media behavior under various loading conditions. However, accurate DEM simulations require meticulous calibration of input parameters, such as particle density, stiffness, and friction, to effectively replicate real-world behavior.

Daphnia stress response to environmental concentrations of chloramphenicol-multi-omics approach.

Commonly used medicines, when discarded or improperly disposed of, are known to contaminate freshwater ecosystems. Pharmaceuticals can be toxic and mutagenic, and can modify freshwater organisms, even at environmentally relevant concentrations. Chloramphenicol (CAP) is an antibiotic banned in Europe.

Tandem mass tag-based proteomic analysis of granulosa and theca interna cells of the porcine ovarian follicle following in vitro treatment with vitamin D and insulin alone or in combination.

This study was performed to investigate the proteomic basis underlying the interaction between vitamin D (VD) and insulin (I) within ovarian follicle using the pig as a model. Porcine antral follicles were incubated in vitro for 12 h with VD alone and I alone or in combination (VD + I) or with no treatment as the control (C). In total, 7690 and 7467 proteins were identified in the granulosa and theca interna compartments, respectively.

Thiol-Specific Linkers for the Synthesis of Oligonucleotide Conjugates via Metal-Free Thiol-Ene Click Reaction.

Chemical conjugation of oligonucleotides is widely used to improve their delivery and therapeutic potential. A variety of strategies are implemented to efficiently modify oligonucleotides with conjugating partners. The linkers typically used for oligonucleotide conjugation have limitations in terms of stability or ease of synthesis, which generates the need for providing new improved linkers for oligonucleotide conjugation.