[Induction of differentiation of leukemic cells].

The review is devoted to news facts in support of the conception advanced by R.E. Kavetsky and others scientists on the possibility of normalization of malignant cell properties due to differentiation increase of these cells under the effect of different inducers.

[Isolation and partial characterization of EGF-like growth factor from rat glioma tissue].

Using acid-ethanol extraction, two proteins with Mr=8 and 12 kD were extracted from rat glioma tissue induced with ethylnitrosourea. These proteins were shown to complete for the receptor with [125I]EGF (epidermal growth factor) on A431 cells. The 8 kD protein exhibited a marked mitogenic effect by stimulating DNA synthesis in resting NIH 3T3 cells.

[Production of a cell line of embryonic rat fibroblasts after transfection with the DNA from the early region of simian adenovirus SA7].

Transfection of plasmid DNA, containing coding sequences for the early region of simian adenovirus SA7, into primary cultures of the rat embryonic fibroblasts results in the establishment of a stable cell line REF-1. The cells have been passed more than 40 times (about 160 doublings) without reaching the critical stage. Their growth is substrate- and concentration-dependent.

[Changes in purine metabolism in the macrophages of mice exposed to a new synthetic analog of muramyl dipeptide].

It has been established that N-acetylglucosaminyl-N-acetylmuramyl-L-alanyl-D-isoglutamine (GMDP), a new synthetic analog of muramyl dipeptide, while incubated in vitro with macrophages essentially inhibits 5'-nucleotidase (5-N) activity without any influence on the activity of adenosine deaminase in these cells. The maximal effect was recorded 24 h after co-incubation. As 0.