Publications by authors named "A M Stephenson"
Streptococcus pyogenes (Sp) Cas9 has been widely utilized to edit genomes across diverse species. To achieve high efficiency and specificity as a gene editing enzyme, Sp Cas9 undergoes a series of sequential conformational changes during substrate binding and catalysis. Here, we employed single molecule FRET techniques to investigate the effect of different KCl concentrations on conformational dynamics of Sp Cas9 in the presence or absence of a single-guide RNA (sgRNA).
View Article and Find Full Text PDFObjective: Given the well-accepted health benefits, it is important to identify scalable ways to support people with long-term conditions (LTCs) to remain physically active. This systematic review aimed to evaluate the effect of digital tools on the maintenance of physical activity (PA) amongst this population.
Methods: Electronic databases were searched for randomised controlled trials investigating the effect of digital tools on PA maintenance at least three months post-intervention compared with a non-digital control in participants with long-term conditions.
Article Synopsis
- - T cells are crucial players in the adaptive immune response, targeting pathogens and damaged host cells through a process that involves interaction with antigen-presenting cells.
- - New biomaterial designs are creating artificial platforms that mimic T cell activation processes, enhancing cell therapies by activating T cells outside the body or providing direct treatment options.
- - This review discusses innovative strategies in designing T cell-stimulating platforms, focusing on various methods like bead-based systems, hydrogels, DNA systems, and soluble activators to improve cancer therapy.
We developed a novel, unobtrusive estimate of team multitasking throughput (tMT). We demonstrate it through the quantitative assessment of tMT in distributed dyads when objective performance and purported reliability are manipulated among teammates. In a within-subjects experiment, we investigated the effects of teammates' performance and purported reliability on tMT.
View Article and Find Full Text PDFBackground: During post-stroke motor rehabilitation, patients often receive feedback from therapists or via rehabilitation technologies. Research suggests that feedback may benefit motor performance, skill acquisition, and action selection. However, there is no consensus on how extrinsic feedback should be implemented during stroke rehabilitation to best leverage specific neurobehavioral mechanisms to optimize recovery.
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