Publications by authors named "A M Makarevich"
Cryopreservation of oocytes is an important tool for preserving genetic resources and for farm animals breeding. Processes taking place during vitrification affect oocytes and result in their reduced developmental capacity and lower fertilisation rates of cryopreserved oocytes. Further improvement in cryopreservation techniques is still required.
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- The study aimed to evaluate whether astaxanthin, an antioxidant, could improve the viability of cryopreserved bovine oocytes during the vitrification/warming process.
- During the recovery phase, astaxanthin treatment led to reduced levels of reactive oxygen species (ROS), lipid peroxidation, and enhanced mitochondrial activity in the oocytes.
- Although there was no direct impact on embryo development, astaxanthin improved the quality of blastocysts derived from vitrified oocytes by influencing gene expression related to apoptosis and oxidative stress.
Cells of pre-implantation embryos are equipped with many morphological and functional systems through which they can synthesize specific proteins and effectively ensure the protection of early embryonic development. Here we present evidence for the existence of these systems in morphologically normal and abnormal bovine blastocyst stage embryos in vivo at the ultrastructural and actin cytoskeleton levels. The appearance of organelles in the trophectoderm (TE) and inner cell mass (ICM) cells, responsible for their synthetic activities and their role in the development of early bovine embryos are described.
View Article and Find Full Text PDFVitrification of bovine oocytes can impair subsequent embryo development mostly due to elevated oxidative stress. This study was aimed at examining whether glutathione, a known antioxidant, can improve further embryo development when added to devitrified oocytes for a short recovery period. Bovine in vitro matured oocytes were vitrified using an ultra-rapid cooling technique on electron microscopy grids.
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- Tetraspanin proteins play a crucial role in organizing cellular complexes on the membranes of most nucleated cells, but their specific functions in folliculogenesis and oogenesis in mammals are not fully understood.
- New research has highlighted the distribution of tetraspanins CD9, CD81, CD151, CD82, and CD63 at various stages of follicular development in cattle, indicating possible cooperation between CD9, CD63, and integrin alpha V in ovarian tissues.
- The study also uncovered unique localization patterns of tetraspanins in bovine oocytes and showed that the distribution of these proteins remains unchanged in vitrified oocytes, emphasizing their potential significance in oocyte development and fertilization processes in cattle.