Publications by authors named "A M Kowalik"
Colorectal carcinoma brain metastases (n = 60) were studied using next-generation sequencing and immunohistochemistry. RAS and BRAF mutations were detected in 58.2% and 7.
View Article and Find Full Text PDFUnlabelled: Pyroptosis is an inflammatory immune response of eukaryotic cells to bacterial lipopolysaccharide (LPS) and other pathological stimuli, leading to the activation of the gasdermin D (GSDMD) and secretion of pore-forming domain GSDMD, facilitating the release of cytokines. Additionally, GSDMD exhibits antibacterial properties through interactions with bacterial outer membranes (OM). We explored alternative antimicrobial strategy to determine whether inducing natural pyroptosis via GSDMD activation by LPS could enhance the effectiveness of recombinant phage endopeptidase KP27 (peptidoglycan-degrading enzyme) against , enabling penetration through OM and bacterial killing synergistically.
View Article and Find Full Text PDFIntroduction: This study aimed to assess the association between the urinary lactate-to-creatinine ratio (ULCR) and brain spectroscopy (1H-MRS) findings in very low gestational age (VLGA) infants with and without preterm brain injury.
Methods: Urine samples were collected from 54 VLGA infants during the first week of life, after 1 month of life, and at term-equivalent age (TEA). Urinary lactate was measured via highly selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) with a quantitative organic acid analysis kit and expressed as the ULCR.
Objective: Magnetic resonance spectroscopy (H-MRS) may provide clinically relevant data regarding metabolic processes that govern the course of preterm brain injury.
Study Design: 46 very preterm infants (VP) were evaluated by magnetic resonance imaging and H-MRS at term-equivalent age. Brain injury was assessed according to the Kidokoro scale.
Article Synopsis
- Treatment of Limbal Stem Cell Deficiency (LSCD) can be effectively managed through autologous transplantation of a patient's own stem cells, a method approved by the European Medicines Agency.
- A study explored using 3D bioprinting to enhance the control and reproducibility of seeding Limbal Epithelial Stem Cells (LESCs) on a scaffold compared to traditional pipetting methods.
- Results indicated that bioprinting allowed for more uniform cell distribution, higher cell viability, and improved epithelial growth rates, suggesting that this technique could optimize LSCD therapy and reduce the number of cells needed for successful treatment.