Publications by authors named "A M Kayastha"

Introduction: Colonic bezoars are undigested or partially digested materials that accumulate in the form of a mass in the colon. There are occasional reports of constipation and intestinal obstruction due to geophagia or pica. Bezoars are due to pica, which is commonly associated with iron deficiency anemia and psychiatry disorders.

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Recent advancements in nanomaterials have significantly impacted various sectors, including medicine, energy, and manufacturing. Among these, organic/inorganic nanohybrids have emerged as transformative tools in the food industry. This review focuses on the innovative applications of these nanohybrids in food packaging, enzyme immobilization, and contamination detection.

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Bioactive urease from watermelon (Citrullus lanatus) seeds was purified using acetone fractionation, anion-exchange, and size-exclusion chromatography, achieving a 121-fold increase and specific activity of 3216 U/mg. The enzyme appeared as a single band on native and SDS-PAGE, with a molecular mass of 480 ± 10 kDa and subunit mass of 80 ± 10 kDa, indicating six identical subunits. Atomic absorption spectroscopy revealed 1.

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Urea contamination in milk poses significant health risks, including kidney failure, urinary tract obstruction, fluid loss, shock, and gastrointestinal bleeding. This highlights the need for sensitive, rapid, and reliable methods to detect traces amount of urea in milk. In this study, we designed an electrochemical transducer for urea detection by utilizing purified watermelon urease (Urs), gold nanoparticles (AuNPs), and graphene oxide (GO).

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Non-heme iron oxygenases constitute a versatile enzyme family that is crucial for incorporating molecular oxygen into diverse biomolecules. Despite their importance, only a limited number of these enzymes have been structurally and functionally characterized. Surprisingly, there remains a significant gap in understanding how these enzymes utilize a typical architecture and reaction mechanism to catalyze a wide range of reactions.

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